Yesterday was Thursday, which means Bio practical on the first 2 periods. We are supposed to make alginate beads and the whole class were so excited, even before this (when the lecturer told us we're doing alginate beads on Monday). She warned us not to play with the beads as some may have allergy to them, causing their hands to itch upon contact, so some girls in the class brought disposable gloves. Yes, they bought them and brought it to the lab (for those who think I'm pointing out them, please don't be offended)...
I took some pictures when doing the experiment, of course without the lecturer looking. First, we mix yeast together with the same amount of glucose solution. Next, we extract 3cm³ of the mixture, then add same amount of alginate. After stirring, add the mixture into a 3cm depth of calcium chloride using a glass rod, then, yeast-glucose alginate beads are formed.
Yeast-glucose Alginate BeadsWe did another one using distilled water instead of glucose solution.
Here is what happened when I just pour the mixture of yeast-distilled water into calcium chloride:
We are supposed to place an alginate bead into hydrogen peroxide solution to observe the activity of enzyme in yeast. Oxygen bubbles are formed around the beads and are used for the enzyme catalase to catalyse amylase. The beads will later float like this:
We also add the alginate beads in bromothymol blue solution. After 15 minutes, one of them turns from blue to yellow (pH6) and the other blue to green (pH7).
When the lab hours is over, some girls collected the beads and took them home. I wanted to take them home too, but still discarded them later since they will disappear eventually.
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Since I posted so much pictures today, so I decided to post 1 more. This was what I cooked last Sunday (at last I moved my lazy hands and decided to cook). It was the second time I cooked myself this year xD. For 2 long months and I cooked twice only, the first time was the day before, Saturday night.
Stopping here now. Good night my blog!!
By,
Blue-Wingz